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| Registros recuperados: 174 | |
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| Abdelbaset, Abdelbaset Eweda; Alhasan, Hend; Salman, Doaa; Karram, Mohamed Hassan; Rushdi, Mahmoud Abd Ellah; Xuan Xuenan; Igarashi, Makoto. |
| Cats are the only definitive hosts of Toxoplasma gondii and constitute an essential source of infection to all warm blooded animals and humans. Diagnosis of T gondii infection in cats is fundamental for proper management and control of infection in humans and animals. In the current study, we have evaluated the diagnostic performance of tachyzoite lysate antigen (TLA) and different T. gondii recombinant antigens including surface antigen 2 (SAG2), dense granule proteins 2, 6, 7, 15 (GRA2, GRA6, GRA7, GRA15) and microneme 10 protein (MIC10) in immunoglobulin G enzyme linked-immunosorbent assay (IgG ELISA) using cat serum samples, with reference to latex agglutination test (LAT). Remarkably, TLA showed better performance than other recombinant antigens in... |
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Palavras-chave: ELISA; Recombinant proteins; Serodiagnosis; Toxoplasmosis. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4574 |
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| Albano,Ana Paula Neuschrank; Klafke,Gabriel Baracy; Brandolt,Tchana Martinez; Da Hora,Vanusa Pousada; Nogueira,Carlos Eduardo Wayne; Xavier,Melissa Orzechowski; Meireles,Mário Carlos Araújo. |
| Paracoccidioides brasiliensis is the etiological agent of the major systemic mycosis in Brazil, called paracoccidioidomycosis. Although the Rio Grande do Sul is considered an endemic area of the disease, there are few studies on the ecology of P. brasiliensis in the state. Therefore, this study aimed to evaluate the infection of P. brasiliensis in horses from the mesoregion of Southwest Riograndense, using these animals as sentinels. Serological techniques, such as double immunodiffusion in agar gel (AGID) and indirect ELISA, were performed to detect the anti-gp43 P. brasiliensis antibody in horses from five different farms in the region of Bagé, RS, Brazil. Serology was performed in 200 Pure Blood English horses up to two years of age that were born and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ELISA; Gp-43; Protein G-peroxidase; Paracoccidioidomycosis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200513 |
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| Almeida,V.M.A.; Gonçalves,V.S.P.; Martins,M.F.; Haddad,J.P.A.; Dias,R A.; Leite,R.C.; Reis,J.K.P.. |
| Estimaram-se, no estado de Minas Gerais, a prevalência e a distribuição espacial da anemia infecciosa eqüina (AIE) em propriedades com eqüídeos de serviço. As amostras de sangue, de 6540 eqüídeos de 1940 rebanhos foram coletadas no período de setembro de 2003 a março de 2004, nos 853 municípios do estado. Utilizaram-se dois testes de laboratório em seqüência: ELISA, usando-se antígeno recombinante gp90, e imunodifusão em gel de ágar (IDGA). As prevalências foram de 5,3% [IC=4,3 a 6,3%] para rebanhos e de 3,1% [IC=2,2 a 3,9%] para animais. O estado de Minas Gerais foi considerado área endêmica para AIE. As mais altas prevalências para rebanhos e para animais foram encontradas na região Norte/Noroeste, seguida pela região Vale do Mucuri/Jequitinhonha. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eqüídeos; Anemia infecciosa; ELISA; IDGA. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000200001 |
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| Álvarez,J; Miguel Cotes,J; Marín,M. |
| La virosis del tomate de árbol en Colombia es causada por un complejo de al menos siete virus, que incluyen miembros de los géneros Potyvirus, Cucumovirus, Polerovirus y Tobamovirus, entre otros. La propagación de este frutal se realiza por semilla sexual, por lo cual es fundamental garantizar su sanidad, especialmente de virus como PVY y PLRV, que han sido informados y pueden ser transmitidos por esta vía. En la investigación se realizaron los análisis filogenéticos de aislados de PVY y PLRV obtenidos a partir de muestras de semilla sexual y plántulas de vivero de tomate de árbol procedentes de diferentes regiones de Colombia. También se desarrollaron técnicas de hibridización molecular con sondas dirigidas al gen de la cápside, como herramientas de... |
| Tipo: Journal article |
Palavras-chave: ELISA; Hibridización molecular; PLRV; PVY; Solanum betaceum. |
| Ano: 2011 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522011000200003 |
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| Alves,L.A.O.; Teixeira,M.F.S.; Pinheiro,A.A.; Pinheiro,R.R.; Dias,R.P.; Brito,R.L.L.; Lopes Júnior,C.A.F.; Bezerra Júnior,R.Q.; Azevedo,D.A.A.. |
| Este estudo teve como objetivo produzir um antígeno (Ag) a partir de cultura de células de membrana sinovial caprina (MSC) infectadas com o vírus de artrite encefalite caprina (CAEV), pela técnica de microfiltração seriada, substituindo a ultracentrifugação em colchão de sacarose (UCCS) para utilização em ELISA indireto (ELISA-i). Amostras de 188 soros caprinos, que previamente foram testados pelo Western blot (WB) com Ag UCCS, foram submetidas à análise pelo ELISA-i com o novo antígeno produzido, que mostrou concordância de 92% em relação ao antígeno UCCS. A sensibilidade e a especificidade do ELISA em relação ao WB foram de 95,6% e 88,5%, respectivamente. A nova técnica, criada a partir de microfiltrações, mostrou-se efetiva e de baixo custo para o... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Caprino; CAEV; ELISA; Lentivírus de pequenos ruminantes; Western blot. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400021 |
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| Aradaib,Imadeldin Elamin; Abbas,Babiker; Osburn,Bennie; Bushara,Hamid Omer; Taylor,Martin. |
| Six calves were immunized with schistosomula of Schistosoma bovis irradiated at 3 or 20 Kilorad (Krad) and three calves were kept as controls. Twenty four weeks post immunization, three calves (one from the 20 Krad and two from the 3 Krad group) were challenged with normal cercaria of S. bovis. The immune response was monitored by agar gel immunodiffusion (AGID) and the Enzyme Linked Immunosorbent Assay (ELISA) using adult worm antigen. Using AGID, precipitin lines were observed only with sera from challenged animals. Using ELISA, the immune response of the vaccinated calves was first detected by 2-3 weeks, peaking by 6-8 weeks post vaccination. The immune response of the three challenged calves was elevated by 2 weeks post challenge, peaking at 8-10 weeks... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ELISA; Schistosoma bovis; Vaccine. |
| Ano: 1994 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781994000300020 |
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| AYALA VÁSQUEZ,MARILUZ; GONZÁLEZ JAIMES,PAOLA; GUTIÉRREZ,PABLO ANDRÉS; COTES,JOSÉ MIGUEL; MARÍN MONTOYA,MAURICIO. |
| El tomate de árbol (Solanum betaceum) es una fruta andina altamente nutritiva, con propiedades organolépticas únicas para el procesamiento industrial y el mercado internacional. En Antioquia, Colombia, este cultivo presenta diferentes problemas fitosanitarios, siendo especialmente limitante la Virosis, causada por un complejo viral del que hacen parte entre otras, especies del género Potyvirus. En este trabajo se evaluaron mediante pruebas de ELISA los niveles de incidencia de este grupo viral en cultivos de siete zonas del departamento de Antioquia y se determinó la identidad taxonómica de dos de los potyvirus asociados al cultivo. Las pruebas serológicas muestran la presencia de potyvirus en seis de las siete zonas evaluadas, alcanzando niveles... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ELISA; Potyvirus; PVY; RT-PCR; Tamarillo. |
| Ano: 2010 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2010000300011 |
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| Aziz,Fariha; Tasawar,Zahida; Lashari,Mushtaq Hussain. |
| ABSTRACT: Cystic echinococcosis is a parasitic infection of worldwide distribution, which, despite causing significant loss of health and money, is still a neglected disease. The present study was performed to determine the prevalence of bovine hydatid disease at Research Centre for the Conservation of Sahiwal Cattle, Jahangirabad, Khanewal, Pakistan. Five hundred bovine serum samples were tested for the detection of bovine Echinococcus antibodies (IgG), using the ELISA kits. Antibodies of bovine Echinococcus were detected in 24.2% cattle. Female cattle showed higher disease prevalence (33.2%) compared to (6.02%) in male cattle. There was positive association between sex of cattle and prevalence (P<0.05). Positive correlation between disease prevalence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Echinococcus antibodies; Seroprevalence; ELISA; Age; Sex. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000400501 |
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| BALDASSI,L.; BARBOSA,M. L.; BACH,E. E.; IARIA,S. T.. |
| Clinical samples from 71 bovine from different Brazilian states were processed for the analysis of anaerobe organisms with emphasis on the isolation and characterization of Clostridium spp. From these, eighty-nine Clostridium perfringens strains were recovered: 32 from liver, 19 from intestinal contents, 14 from kidney, 6 from rumen, 5 from nervous system, 4 from bone marrow, 2 from udder tract, blood, spleen and lung, and one from muscle. Four reference Clostridium perfringens types A, B, C, and D were used as controls in this study. All isolates were cultivated in appropriate media, and after centrifugation the supernatant and sediment were separated. From pure supernatant post exopolysaccharide (EPS) extraction, mouse toxigenicity tests were performed,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium perfringens; Toxigenicity; Bovine isolates; ELISA. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100010 |
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| Bannai, Hiroshi; Nishikawa, Yoshifumi; Seo, Jin-yong; Nakamura, Chinatsu; Zhang, Sofa; Kimata, Isao; Takashima, Yasuhiro; Li, Junyou; Igarashi, Ikuo; Xuan, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南. |
| An enzyme-linked immunosorbent assay (ELISA) based on the recombinant p23 of Cryptosporidium parvum was established for the detection of antibodies against C. parvum in cattle. The sensitivity and specificity of the ELISA were evaluated with the standard indirect fluorescent antibody test (IFAT) using sporozoites as antigens. Of 77 bovine sera collected from China, 20 (26.0%) were identified as positive by the IFAT. The same samples were tested with the ELISA. The optical densities at 415 nm were compared to the IFAT results and statistically analyzed to designate a provisional cut-off point. As a result, the cut-off point was concluded to be 0.08, which was considered to be the best condition in the light of its sensitivity (80%) and specificity (73.7%).... |
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Palavras-chave: Cryptosporidium parvum; P23; ELISA; IFAT. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/142 |
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| Barbosa da Silva,Jenevaldo; Baêta,Bruna de A.; Lopes,Cinthia T.A.; Martins Land Manier,Bruna Sampaio; Nunes Santana de Castro,Gustavo; Nunes dos Santos,Priscilla; da Fonseca,Adivaldo Henrique; Diomedes Barbosa,José. |
| Sera samples were collected from 330 water buffaloes on Marajó Island, state of Pará, Brazil, to assess the presence of antibodies against Borrelia burgdorferi by indirect Enzyme Linked Immunosorbent Assay. Approximately 45% of the animals had antibodies against B. burgdoferi. The prevalence of seropositive buffaloes, 72% (85/118), was statistically higher in the city of Soure than in the other municipalities tested. Murrah breed animals were significantly more seropositive (Prevalence Ratio (PR) = 1.84, p = 0.000) than those of the Mediterranean breed. Among the animals diagnosed positive for tuberculosis, 33% (4/12), were also seropositive for B. burgdoferi. Animals positive for tuberculosis had a significantly lower level of B. burgdorferi... |
| Tipo: Journal article |
Palavras-chave: Spirochetes; Borrelia sp.; ELISA; Buffaloes; Risk factors; Espiroquetas; Borrelia sp.; ELISA; Búfalos; Factores de riesgo. |
| Ano: 2014 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000300002 |
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| Barbosa da Silva,Jenevaldo; Nunes de Santana Castro,Gustavo; Nunes dos Santos,Priscilla; Henrique da Fonseca,Adivaldo; Henrique da Silva Lima,Danillo; dos Anjos Bomjardim,Henrique; dos Santos Belo Reis,Alessandra; de Oliveira Soares,Susiane; Diomedes Barbosa,José. |
| The aim of this study was to determine the prevalence of Toxoplasma gondii in cattle from the Northern and Midwestern regions of Brazil. Serum samples were collected from 1789 animals and tested by indirect enzyme-linked immunosorbent assay (ELISA). The overall prevalence of T. gondii was 83.40% (1492/1789). The prevalence rates of T. gondii-seropositive animals observed in the states of Pará, Tocantins and, Mato Grosso were 87.45%, 87.79% and 73.06%, respectively. The detection of high prevalence rates of T. gondii in cattle deserves special attention because they are the main source of high biological value protein for humans. This finding indicates the need for further studies on the risk that these animals may pose to public health. |
| Tipo: Journal article |
Palavras-chave: Cattle; ELISA; IFAT; Toxoplasma gondii. |
| Ano: 2015 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2015000100008 |
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| Barbosa,Celso Guimarães; Sanavria,Argemiro; Freire,Ronald Bastos. |
| Six bovines were infested with 60 first instar larvae of Dermatobia hominis. The animals were bleed weekly, and their antibodies levels to D. hominis L1, L2 and L3 instars measured during the time, following the infestation course. The antisera were submitted to a titration against optimal dilutions of antigen coated wells of microplates, previously sensitized with L1, L2 and L3 preparations, respectively. The ELISA assay was used to test single dilutions of antisera, which results were comparatively analyzed with a control of not infested animals. Antibodies against L1 were detected between the first and 21st day post-infestation (DPI) and, from the 42nd DPI on. Anti-L2 antibodies, could be detected on the 21st DPI and from the 35th DPI until... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dermatobia hominis; Myiasis; Humoral immune response; ELISA. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000300013 |
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| Registros recuperados: 174 | |
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